FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115426
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19250.047.8804933
CCGAGTA19850.045.3511542
TACTCGG18500.043.9831283
AGTAGCT72500.042.1067855
CCCGAGT27800.039.7031481
CTACTCG18550.039.599992
GAGTAGC47900.038.1832924
TTGGGAG112300.037.69836
GTAGCTG77250.037.4672476
GCTGGGA141850.035.9358679
TAGCTGG85250.035.133647
GGAGGCT119550.035.1109969
GGGAGGC148950.033.69128
GCTACTC47050.032.965311
TCGGGAG24500.032.913666
CTCGGGA23500.032.7823525
CGGAGGT21600.032.3327187
TGGGAGG152050.030.0211327
GGAGGTT58750.029.5347568
GCACTTT108500.029.1220991
Produced by FastQC (version 0.11.5)