FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115426
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA17250.046.747132
GAGTAGC43300.045.7263534
CGAGTAG16700.045.2687873
AGTAGCT69000.044.2423825
CGGAGGT26850.043.9765667
TTGGGAG111350.041.8346186
GCTGGGA146850.041.723189
TACTCGG20600.040.8925933
CTACTCG20900.039.961132
GTAGCTG76850.039.1610376
GGAGGCT124700.037.644629
TAGCTGG86250.036.9799587
GGGAGGC151500.036.3080148
GGAGGTT65250.036.0818678
CCCGAGT25850.035.100091
AGGCGGA36550.035.0634274
GCTACTC51850.034.5818941
CTCGGGA23800.033.8815165
GCGGAGG36000.032.9991846
TCGCGTT1751.0741314E-732.913475
Produced by FastQC (version 0.11.5)