FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5917066
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21200.051.9613652
CGAGTAG21400.049.1206443
TTGGGAG126250.043.2847946
TACTCGG22700.043.1358153
GAGTAGC51900.042.033954
CCCGAGT28300.040.712891
AGTAGCT81850.039.671885
CTACTCG23250.039.638022
GCTGGGA167450.038.5254559
GGAGGCT145650.037.02519
GGGAGGC174350.036.629138
GTAGCTG89800.036.560616
CGGAGGT30300.035.8805667
GGAGGTT73700.035.364388
TAGCTGG99350.034.2057467
TGGGAGG172200.034.0342527
CTCGGGA27300.034.0213855
TTTGGGA142950.032.5366865
TCGGGAG29300.032.1905676
GCACTTT125550.031.4886461
Produced by FastQC (version 0.11.5)