FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6189828
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG24100.037.6457523
AGTAGCT91150.034.8341985
CGAGTAG24200.034.812323
CTACTCG22700.034.2628562
CCGAGTA25250.033.6547432
TTGGGAG130450.032.9499056
GTAGCTG96900.032.4699486
GCTGGGA179550.032.3201649
GAGTAGC58100.032.0983474
GGAGGCT148400.031.6328019
TAGCTGG106800.030.4038937
GCTACTC61050.030.0133691
GGGAGGC175300.029.5305088
CCCGAGT33850.028.7701281
CTCGGGA29200.028.3556065
TCGCGTT2105.3057374E-727.4280325
TGGGAGG177100.026.9943857
TCGGGAG32600.026.9442526
ACTCGGG35650.025.6509324
GGAGGTT73650.025.612538
Produced by FastQC (version 0.11.5)