FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6189828
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21300.039.549113
CTACTCG26300.038.600582
CCGAGTA22300.038.4213452
TACTCGG26750.038.2203833
AGTAGCT89250.034.527575
TTGGGAG139950.033.749046
GCTACTC61900.033.1549071
GCTGGGA176350.033.0296139
GAGTAGC57450.032.8353584
GGAGGCT153350.032.631089
GTAGCTG95150.031.705586
TCGGGAG36250.031.3819226
GGGAGGC187000.030.8404968
GGAGGTT78050.030.6263778
CTCGGGA33350.030.4406385
CCCGAGT32050.030.1071991
TAGCTGG105900.029.8468867
ACTCGGG36750.028.2121144
CGGAGGT30450.027.4284427
GAGGTTG95550.027.2023839
Produced by FastQC (version 0.11.5)