FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5745980
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21450.038.6057623
AGTAGCT79750.038.2789575
CTCGCGT2900.037.2452664
CCGAGTA23000.036.9487762
GAGTAGC53800.036.8068124
CGGAGGT25450.036.494457
TTGGGAG122850.035.750296
GCTGGGA160650.035.002199
GGAGGTT73300.034.6733368
GTAGCTG88350.033.982436
GGAGGCT133900.033.8216639
GGGAGGC165250.032.5464828
TAGCTGG97900.031.4764677
CCCGAGT31950.031.3799211
TACTCGG22500.031.3635463
TGGGAGG158550.031.1063617
GAGGTTG91900.030.6327639
CTACTCG22250.029.778562
AGGCGGA37800.029.7173864
GCTACTC57000.029.104551
Produced by FastQC (version 0.11.5)