FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4423551
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA16600.053.7991372
CGAGTAG16600.052.923633
AGTAGCT67800.049.37965
GAGTAGC42800.048.4548034
GTAGCTG71100.046.4801336
CGGAGGT20600.044.387517
CCCGAGT22300.043.671031
GCTGGGA124350.043.3091939
TAGCTGG79500.043.0180557
TACTCGG18950.041.4204833
TTGGGAG98350.040.4100156
GGGAGGC128550.037.8615958
CTACTCG19400.037.4956932
TCGGGAG22700.037.4266056
CTCGGGA21750.036.7441335
GGAGGCT101100.036.462229
AGGCGGA29750.035.5810284
GGAGGTT54800.035.3423968
TGGGAGG128550.034.6131177
GGCGGAG31500.033.8279345
Produced by FastQC (version 0.11.5)