FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325893
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA24700.041.4041752
CGAGTAG23650.040.801063
CGGAGGT24950.037.514157
AGTAGCT90150.036.4184465
GAGTAGC59050.035.973794
GCTGGGA170250.034.846769
CCCGAGT34550.034.2453161
GTAGCTG96650.033.3732456
TAGCTGG104650.031.2348147
TTGGGAG128650.031.1162536
GGAGGCT137000.030.848919
TACTCGG23750.030.0171453
GGAGGTT74350.029.9226328
CTACTCG24400.029.2212052
GGGAGGC165200.028.6772948
AGGCGGA39050.028.0289364
GGCGGAG41400.027.6515255
GCTACTC59850.027.4837571
GCGGAGG36550.027.184046
GAGGTTG93200.027.0379929
Produced by FastQC (version 0.11.5)