FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325893
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA22550.045.9768832
CGAGTAG22300.043.909413
CTACTCG24600.038.6333542
CCCGAGT31150.038.6056331
TACTCGG24650.038.5549933
GAGTAGC56350.037.6922154
AGTAGCT92150.037.4251675
GCTGGGA174350.036.75299
TTGGGAG135100.036.0259066
TCGCGTT2451.8189894E-1235.2645845
GGAGGCT145650.034.5039259
GTAGCTG96800.034.2141726
TAGCTGG101550.033.96097
CGGAGGT28350.033.523127
GCTACTC63350.033.1916241
GGGAGGC174750.033.1667028
CTCGGGA28600.032.9783445
TCGGGAG32800.030.9505836
TGGGAGG181750.030.383977
GGAGGTT73500.029.485118
Produced by FastQC (version 0.11.5)