FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5030036
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA16500.040.596052
GCTGGGA145650.040.5838629
AGTAGCT74400.039.579575
CGAGTAG16350.039.1982233
GTAGCTG77350.038.814726
TACTCGG20700.038.2662963
CGGAGGT21400.038.017667
TTGGGAG111250.037.794936
TAGCTGG86400.036.2490167
CTACTCG21900.036.177072
GAGTAGC47950.035.4415864
TCGGGAG25050.035.0649156
CTCGGGA23350.034.226055
GGAGGCT115400.033.0043039
GGAGGTT61050.032.667428
GGGAGGC145900.032.4213268
CCCGAGT22750.032.3434561
GCTACTC51100.031.1988851
TAGCGCG1652.5219433E-630.5446265
GTGCTGG96250.029.9212657
Produced by FastQC (version 0.11.5)