FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4534111
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13700.041.5173722
CGAGTAG13450.039.6125453
CGGAGGT19800.038.181017
TTGGGAG88100.036.1218226
CTACTCG14400.035.4992522
GCTGGGA108750.034.8902979
CCCGAGT18850.034.7628671
TACTCGG15700.034.3941763
GGAGGCT95850.033.80219
GAGTAGC35100.033.4351924
AGTAGCT57150.033.3851245
AGGCGGA27700.033.2700544
GGAGGTT54150.033.2402958
GGGAGGC111400.033.0908628
GGCGGAG29350.032.6262255
TCGCGTT1551.5482201E-632.515445
GAGGTTG64150.032.435799
CTCGGGA17800.031.954385
GTAGCTG59750.031.932386
TAGCTGG66950.031.6169557
Produced by FastQC (version 0.11.5)