FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6426086
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA28600.029.9584832
CGAGTAG27900.029.4193293
AGTAGCT111100.027.9300465
GAGTAGC66750.027.3977324
GTAGCTG118300.025.925876
CTACTCG29600.024.8111762
GCTGGGA218600.024.800099
TAGCTGG129200.024.6302177
CCCGAGT39100.024.2742271
TACTCGG30750.023.4146163
GCTACTC68600.021.017441
TCGGGAG38700.020.4639846
TTGGGAG146950.020.3813156
CTCGGGA35350.020.3666235
CAAGTAG56000.020.0571253
GGAGGCT173450.019.840939
CCCAAGT75950.019.555861
CCAAGTA62600.019.2079522
AAGTAGC61150.018.6034184
AGCTGGG187400.018.517658
Produced by FastQC (version 0.11.5)