FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5553244
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG22550.036.4005782
TACTCGG22750.034.8145873
AGTAGCT80400.031.6097975
GCTGGGA150800.029.456899
GCTACTC52150.029.3125421
CGAGTAG19150.028.5755633
CTCGGGA27200.028.321615
GTAGCTG84450.028.2183346
CCGAGTA19750.028.0720162
TTGGGAG109700.027.9579966
GAGTAGC52550.027.4035764
TAGCTGG91450.027.0030927
CGGAGGT22850.026.7815557
GGAGGCT129250.026.2357449
TCGGGAG29400.026.2023076
GGGAGGC148200.025.6500848
ACTCGGG30950.024.660124
CGTACGG1550.001883408423.2267422
GGAGGTT60900.023.0526248
CCCGAGT29050.022.6944981
Produced by FastQC (version 0.11.5)