FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5098392
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18550.044.220973
CCGAGTA20100.044.032812
CGGAGGT22150.041.9140857
AGTAGCT73350.041.692335
GAGTAGC47800.041.5478484
TTGGGAG109050.039.5246936
GCTGGGA142150.038.9841359
GTAGCTG81150.037.241586
TAGCTGG85750.036.1732187
CCCGAGT26350.035.7732161
GGAGGTT60650.035.717398
GGAGGCT118450.035.6046379
GGGAGGC140400.035.0104458
TACTCGG20050.033.0172423
TGGGAGG144700.032.6271637
AGGCGGA34000.032.5918584
CTCGGGA21850.032.2731975
TCGGGAG25000.031.9484886
GGCGGAG36450.031.7830585
CTACTCG19500.031.365462
Produced by FastQC (version 0.11.5)