FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427637
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19700.046.0495343
CCGAGTA20350.045.640482
AGTAGCT78800.040.383225
GAGTAGC48650.038.4779054
CCCGAGT27750.038.129821
GTAGCTG84900.037.312126
TACTCGG23700.037.06233
GCTGGGA155250.036.9135939
TTGGGAG110950.036.1437156
TAGCTGG90750.035.700217
CTACTCG22350.035.4356232
GGAGGCT128000.034.3665669
CGGAGGT26250.033.1864627
GCTACTC57150.032.717371
GGGAGGC154300.031.6350618
TCGGGAG30150.029.610066
CTCGGGA28050.029.516845
TGGGAGG153400.029.2862747
AGGCGGA35800.028.5578984
GGAGGTT66150.028.079858
Produced by FastQC (version 0.11.5)