FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427637
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG20500.047.7353
CCGAGTA21350.046.171562
GAGTAGC50850.041.3184284
TTGGGAG119150.040.339856
TACTCGG21000.040.0883753
GCTGGGA159250.039.9940839
AGTAGCT81500.039.9937825
CCCGAGT28600.038.2410161
CTACTCG22200.037.597322
GTAGCTG85400.037.3248186
GGAGGCT134100.035.366219
TAGCTGG93850.034.967337
GGGAGGC162100.034.540498
CTCGGGA26300.034.1984255
TCGGGAG28050.033.860476
TGGGAGG163500.032.660147
CGGAGGT27650.031.2333137
TTTGGGA125200.030.6319565
GGAGGTT67500.030.4925798
GCACTTT111650.029.902751
Produced by FastQC (version 0.11.5)