FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4803607
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24400.061.081163
AGTAGCT92550.060.4440045
CCGAGTA24950.059.7359242
GAGTAGC58400.058.9307254
GTAGCTG99000.054.5424586
GCTGGGA198200.052.1626249
TTGGGAG147400.051.2861986
CCCGAGT31850.051.1690141
TAGCTGG113150.049.630517
GGAGGCT156400.044.376089
CTACTCG26100.044.1382642
TACTCGG25600.043.593173
TTTGGGA145850.042.4028855
GCACTTT127800.042.053971
GGGAGGC207400.040.7885068
GGAGGTT76400.040.3328678
GCTACTC65500.039.920861
CGGAGGT32000.039.8228157
AAGTGCT123550.039.508965
TGGGAGG202100.039.2219967
Produced by FastQC (version 0.11.5)