FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084231
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24400.061.6730653
CCGAGTA26250.060.618062
AGTAGCT96950.060.2257465
GAGTAGC59350.059.808824
GTAGCTG102650.056.2502676
GCTGGGA212400.048.7092489
TTGGGAG145500.048.541726
TAGCTGG124200.048.4611637
CCCGAGT34600.047.1078451
CGGAGGT33700.041.2322047
GCACTTT128350.040.4678421
GGAGGCT161050.040.4126059
CAAGTAG50500.039.4936643
CTACTCG28750.039.0684132
GGAGGTT79900.039.0166748
TACTCGG29300.038.8265273
GGGAGGC213450.038.822938
TTTGGGA147750.038.544125
AAGTAGC55000.037.964214
CCAAGTA53500.037.9519732
Produced by FastQC (version 0.11.5)