FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4191901
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21100.061.0417863
CCGAGTA22200.059.637792
AGTAGCT78250.057.6553845
GAGTAGC51250.057.1422354
GTAGCTG82250.054.151616
TAGCTGG92150.049.279747
GCTGGGA158700.048.7490239
TTGGGAG116400.048.031346
CCCGAGT27550.047.0118751
GGAGGCT130950.043.9661949
TACTCGG20950.042.2452353
CTACTCG20950.041.9017752
GGGAGGC164050.040.315658
TTTGGGA114750.039.7551355
GCACTTT102150.039.4463041
GCTACTC55050.038.558611
CGGAGGT28200.038.2804077
TCGGGAG26850.036.7141046
TGGGAGG161750.036.5731127
AAGTGCT95950.036.370855
Produced by FastQC (version 0.11.5)