FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4519353
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT81500.054.59365
GAGTAGC51500.052.987294
CGAGTAG21450.052.7001723
CCGAGTA21650.052.2144932
GTAGCTG87000.049.5699426
GCTGGGA159650.048.1178679
TAGCTGG98400.045.729437
CCCGAGT27050.044.177481
TTGGGAG114700.044.0640686
CGGAGGT25500.041.7861567
GGAGGCT126150.040.5782439
TACTCGG20650.039.4001663
GGAGGTT66150.038.311028
CTACTCG21100.037.5372
GGGAGGC153200.036.938118
GCTACTC54350.035.845691
CAAGTAG43100.035.5829543
AAGTAGC46550.034.9565434
TTTGGGA116050.034.6178365
TCGGGAG27600.034.433076
Produced by FastQC (version 0.11.5)