FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988578
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA20000.049.6796042
CGAGTAG19500.047.630393
AGTAGCT75750.046.0963365
GTAGCTG81500.041.60746
CCCGAGT27100.041.4292261
GAGTAGC46350.041.320064
GCTGGGA152850.040.4137469
TTGGGAG110800.039.1818436
TAGCTGG89400.038.97767
CGGAGGT27050.038.060647
TACTCGG20750.037.821393
GGAGGTT62650.037.2332738
GGAGGCT121900.037.090549
CTACTCG21100.036.1703262
GCTACTC50400.035.929721
GGGAGGC145400.034.3143778
TGGGAGG147950.032.1657567
TCGGGAG25550.032.1233836
CTCGGGA24850.031.8693775
AGGCGGA38450.031.6460444
Produced by FastQC (version 0.11.5)