FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4286145
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA20900.059.906652
AGTAGCT75650.056.9757465
CGAGTAG21500.056.896113
GAGTAGC46950.055.634364
GTAGCTG82550.051.6903766
TACTCGG20250.050.8139653
CCCGAGT27750.049.0084421
GCTGGGA160050.048.9233559
CTACTCG21700.047.4185642
TTGGGAG121550.047.1227076
TAGCTGG95000.046.4386867
TCGGGAG27300.042.963246
GGAGGCT124850.041.4460759
GGGAGGC165250.040.45928
CTCGGGA26050.040.0527535
CGGAGGT27700.038.9721647
GGAGGTT64550.038.799498
TTTGGGA122950.037.924395
AAGTGCT104500.037.8720255
GCTACTC51900.037.4341931
Produced by FastQC (version 0.11.5)