FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3815462
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26700.083.004723
GAGTAGC61650.082.051324
AGTAGCT95050.081.758895
CCGAGTA27400.080.358942
GTAGCTG102900.073.843446
TAGCTGG120000.064.592667
GCTGGGA215050.064.222069
CCCGAGT35400.063.8248371
TTGGGAG138150.063.752016
CAAGTAG39400.061.728233
GCACTTT111850.059.2498131
AAGTGCT110850.058.745725
TTTGGGA126550.057.5984275
CTGAGTA48100.056.995852
AAGTAGC44650.056.7263154
ACTTTGG116150.055.8173143
TGAGTAG49050.055.7452623
CCAAGTA43550.055.1850742
CACTTTG120700.053.9516372
GGAGGCT156350.052.0150839
Produced by FastQC (version 0.11.5)