FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5628736
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA23000.045.7035332
CGAGTAG21950.044.281673
AGTAGCT88800.041.2677045
GAGTAGC53300.040.929854
TTGGGAG124550.038.7290276
GCTGGGA173700.038.2981349
TAGCTGG100800.037.0691227
CTACTCG23300.036.7718772
GTAGCTG98400.036.5830886
CGGAGGT28300.036.124967
GGAGGCT138150.035.5417639
CCCGAGT32350.035.150781
TACTCGG24850.035.0577243
GGGAGGC169700.033.8977138
GCTACTC59150.032.8163031
GGAGGTT72700.032.779258
CTCGGGA28200.031.1469975
TGGGAGG172450.030.5600017
TCGGGAG30300.030.176346
TTTGGGA133050.029.2202765
Produced by FastQC (version 0.11.5)