FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4115078
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT119850.085.060995
CGAGTAG36250.083.623213
GAGTAGC79400.080.799734
GTAGCTG125600.079.9631356
CCGAGTA38600.079.837912
GCTGGGA266400.068.238969
TAGCTGG152800.067.660687
TTGGGAG170400.064.7705846
CCCGAGT47550.064.4497451
CAAGTAG49200.063.66153
CCAAGTA53100.062.375792
AAGTGCT135300.060.2356875
AAGTAGC54750.059.9699174
GCACTTT137750.059.589491
TTTGGGA156500.058.2864345
CTGAGTA55600.056.851572
CACTTTG145300.056.1457672
ACTTTGG145750.055.9230163
TGAGTAG57300.055.918833
TACTCGG36800.054.980823
Produced by FastQC (version 0.11.5)