FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4115078
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT115300.083.872755
CCGAGTA30400.083.3143842
CGAGTAG31000.083.094493
GTAGCTG122050.078.8214656
GAGTAGC73750.078.636454
TTGGGAG172900.069.74776
GCTGGGA257200.066.7084969
TAGCTGG149050.066.3921367
CCAAGTA52600.065.524042
CAAGTAG51150.065.2714543
GCACTTT142400.064.980321
TTTGGGA156950.064.916165
ACTTTGG149900.062.1611483
CACTTTG150800.061.408442
CCCGAGT41900.060.619391
AAGTAGC56300.060.451024
CTGAGTA53450.056.8088072
AAGTGCT130000.056.6770865
CTTTGGG169900.056.6648374
TGAGTAG55250.055.9998783
Produced by FastQC (version 0.11.5)