FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882476
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28350.080.764413
AGTAGCT98900.079.7131965
GAGTAGC64750.077.061844
CCGAGTA30850.075.6227652
GTAGCTG108350.073.2924046
TAGCTGG130550.063.365897
GCTGGGA222700.063.1385239
TTGGGAG147650.062.5612756
CCCGAGT39250.060.136171
CAAGTAG44150.060.015453
AAGTAGC46700.056.584194
CCAAGTA49200.055.028222
TTTGGGA138450.054.8620345
GCACTTT118750.053.41071
GGAGGCT167050.052.4944279
TGAGTAG48700.051.5991333
AAGTGCT116950.051.4657635
ACTTTGG129950.050.0885053
CTGAGTA52550.049.1908652
CACTTTG130100.048.4832
Produced by FastQC (version 0.11.5)