FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882476
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG29700.089.641993
CCGAGTA31050.085.281032
GAGTAGC67500.083.25564
AGTAGCT107150.081.659675
GTAGCTG113100.076.854726
CCCGAGT37000.071.3724441
GCTGGGA229500.067.859079
TAGCTGG134250.066.954767
TTGGGAG150100.063.7104266
CCAAGTA47000.058.942572
CAAGTAG46850.057.9025843
AAGTAGC49700.056.6091424
GCACTTT120650.056.538881
AAGTGCT124100.056.358665
TTTGGGA135350.056.299425
ACTTTGG124500.053.8657573
GGAGGCT167400.053.5238349
TGAGTAG51500.053.2333683
CACTTTG125850.052.544652
CTGAGTA53250.052.0244262
Produced by FastQC (version 0.11.5)