FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4706250
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA24350.056.4762762
CGAGTAG23650.056.3212433
AGTAGCT87300.055.0072825
GAGTAGC54500.051.654954
GTAGCTG91700.051.1902056
CCCGAGT30800.048.668751
GCTGGGA179600.046.149
TAGCTGG104100.045.9917037
TTGGGAG123400.045.041286
CTACTCG23750.043.3514982
TACTCGG24150.041.739053
GGAGGCT137350.039.8376359
GGGAGGC171250.038.3838738
CAAGTAG45200.038.2300153
CCAAGTA49350.037.0576822
AAGTAGC48650.035.9629334
GCTACTC57700.035.3918951
TCGGGAG28900.035.3752176
TTTGGGA123200.035.1214455
AAGTGCT109950.034.9012155
Produced by FastQC (version 0.11.5)