FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4706250
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21200.059.735332
CGAGTAG21350.058.3045853
AGTAGCT84500.052.624295
GAGTAGC53650.052.1716234
GTAGCTG87700.049.9657256
TTGGGAG127350.048.477796
CCCGAGT29350.047.0703661
GCTGGGA175050.046.6683129
TAGCTGG102550.043.3751647
CTACTCG25250.041.8900072
TACTCGG25300.041.2384153
GGAGGCT146100.040.052419
GCACTTT111500.039.945721
GGGAGGC188550.039.471388
TTTGGGA127750.039.314165
CGGAGGT30750.038.855397
GCTACTC59300.038.100391
TGGGAGG182100.037.0354777
CACTTTG119500.036.970472
GGAGGTT75500.036.8937078
Produced by FastQC (version 0.11.5)