FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5028364
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA18350.041.198882
CGAGTAG17650.039.9761123
GAGTAGC41950.038.1013374
GCTGGGA127050.037.7403459
AGTAGCT68750.035.6051185
CGGAGGT20300.034.756527
TTGGGAG99200.034.473726
CCCGAGT24950.033.683941
TAGCTGG79050.033.333857
GTAGCTG74200.032.5047766
CTACTCG16450.031.9513442
AGGCGGA29000.031.7783374
GGAGGCT109650.029.9407379
GGAGGTT56850.029.6340948
GGGAGGC126550.029.5833558
TGGGAGG126600.029.34427
GGCGGAG32250.027.905295
TACTCGG18400.027.7817573
TCGCGTT1856.1469054E-627.2415965
CTCGGGA20100.027.2222375
Produced by FastQC (version 0.11.5)