FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5028364
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16100.049.160433
CCGAGTA18550.043.443322
GAGTAGC43750.039.8002854
CTACTCG18300.039.711762
GCTGGGA130950.039.24039
AGTAGCT70800.038.9237255
TACTCGG18900.038.4510733
GTAGCTG75150.036.862156
CCCGAGT24450.036.49151
TAGCTGG81500.036.293087
TTGGGAG100600.035.0466886
CTCGGGA22050.033.61075
GGAGGCT110050.033.0248579
GGGAGGC128400.032.9573178
GCTACTC45250.032.75651
AGGCGGA31450.030.8860264
TGGGAGG134350.029.9978377
CGGAGGT23100.029.908797
TCGGGAG24600.029.834176
GGAGGTT60100.028.8590568
Produced by FastQC (version 0.11.5)