FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4914528
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA144750.041.2828949
CCGAGTA20300.041.14332
CGAGTAG19550.040.8789863
AGTAGCT75250.040.853765
GTAGCTG77500.039.5747836
CGGAGGT22550.038.312937
GAGTAGC48250.038.051064
TAGCTGG88250.038.017357
TTGGGAG112050.036.945936
CCCGAGT26800.036.216691
GGAGGTT59200.034.6604168
GGAGGCT122150.032.122829
GCTACTC50400.031.7614611
TACTCGG21100.031.3927443
CTACTCG20300.031.2121582
GGGAGGC148150.031.199248
AGGCGGA31800.031.0182534
GAGGTTG77450.030.9551899
GGCGGAG32350.030.7125195
TGGGAGG149500.028.8948697
Produced by FastQC (version 0.11.5)