FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4914528
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA18950.046.7056662
CGAGTAG17950.044.898023
GAGTAGC46750.044.0208174
AGTAGCT75800.043.193255
GCTGGGA154500.040.2450039
TTGGGAG105750.039.9421426
CGGAGGT26200.039.553777
CCCGAGT24700.038.7461361
TACTCGG20550.038.517183
GTAGCTG82500.037.8537986
GGAGGTT63300.037.745148
TAGCTGG92700.036.1770257
CTACTCG21450.035.8946842
GCTACTC50800.035.2703131
GGGAGGC143050.035.1654368
GGAGGCT122450.034.4989629
TCGGGAG26200.034.0560346
GAGGTTG83800.033.1489689
TGGGAGG143500.032.748247
CTCGGGA24600.032.1759385
Produced by FastQC (version 0.11.5)