FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5600142
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT75700.034.7888375
CGAGTAG19600.033.7743073
CCGAGTA20500.033.6955152
GAGTAGC48250.033.106284
GCTGGGA141900.032.1044469
TTGGGAG111850.031.3290846
GTAGCTG81100.031.319056
CTACTCG24650.030.9416662
GGAGGCT127050.030.8154269
TACTCGG23950.030.3438423
TAGCTGG89700.030.2477327
GCTACTC56800.029.7696841
GGGAGGC147950.028.8458718
CGGAGGT27500.028.5257957
TCGGGAG28600.028.4293636
CTCGGGA26700.028.2965035
CCCGAGT28950.028.0856571
GGAGGTT66000.027.260898
AGGCGGA36800.027.1782634
TGGGAGG148100.026.1439487
Produced by FastQC (version 0.11.5)