FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5466523
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA20150.038.23462
CGAGTAG20750.037.8223273
AGTAGCT76050.034.7436075
GAGTAGC50300.033.2093124
GCTGGGA135750.032.6699879
GCTACTC52750.031.9944931
TACTCGG22850.031.8254853
GTAGCTG80150.031.4392816
TAGCTGG87150.030.4010457
TTGGGAG105400.030.191856
CCCGAGT26950.030.102391
CTACTCG22500.029.761052
GGAGGCT118150.029.4930529
CTCGGGA25400.027.7779665
GGGAGGC136500.027.5325168
CGGAGGT23050.026.861837
GGAGGTT57100.026.604468
TCGGGAG28000.025.1985846
CCCGAAT11600.024.9772011
AGGCGGA30950.024.6595464
Produced by FastQC (version 0.11.5)