FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5466523
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA20700.041.0161632
CGAGTAG21500.038.4859963
GCTGGGA146500.036.843969
AGTAGCT76000.036.638765
GAGTAGC50750.035.8697054
CGGAGGT23500.035.2186477
CCCGAGT27200.034.9179231
GTAGCTG80900.033.97496
TTGGGAG106500.033.915446
TACTCGG21450.033.8795363
CTACTCG21800.033.6656532
TAGCTGG89200.032.9990277
AGGCGGA32550.032.494494
GGAGGCT116450.032.2606939
GGGAGGC139150.031.290678
GCTACTC51850.031.2231791
CTCGGGA23700.030.0559335
TGGGAGG147300.028.2890597
GGAGGTT64950.028.0339368
GGCGGAG36750.027.8018935
Produced by FastQC (version 0.11.5)