FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6012704
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21700.051.09713
CCGAGTA22450.049.390482
AGTAGCT91350.046.972545
GAGTAGC57400.044.2790264
GTAGCTG94450.043.982536
GCTGGGA179800.042.444619
TAGCTGG106150.041.1016277
CCCGAGT29150.040.257711
TTGGGAG132850.038.585566
CGGAGGT31650.038.2157067
GGAGGCT141100.035.615159
GGAGGTT77700.034.932268
GGGAGGC177500.032.9760448
AGGCGGA41950.032.782094
TACTCGG23000.031.9306373
TGGGAGG178050.031.4993697
GAGGTTG95750.030.9788139
AGCTGGG150250.030.9545428
GGCGGAG44100.030.8552865
GCTACTC59900.030.1123811
Produced by FastQC (version 0.11.5)