FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6012704
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA22800.053.6501922
CGAGTAG22350.053.4426233
AGTAGCT92100.046.797695
GAGTAGC57100.046.37344
GTAGCTG95600.044.1811946
GCTGGGA182200.043.5683449
CCCGAGT29900.043.5576671
TTGGGAG135850.043.167336
TACTCGG24050.040.0910073
TAGCTGG110500.039.9247447
GGAGGCT151650.038.914859
CTACTCG24950.037.2028662
GGGAGGC185000.036.9958238
TGGGAGG186550.035.4539157
CTCGGGA29400.033.7745065
TCGGGAG31350.032.8212976
CGGAGGT31800.032.3633047
TTTGGGA147050.031.9525415
GCACTTT131500.031.900691
GGAGGTT75500.031.7425048
Produced by FastQC (version 0.11.5)