FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867512
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA18700.050.8230552
CGAGTAG19150.049.2528043
AGTAGCT73800.046.046465
CGGAGGT21500.044.8721587
GAGTAGC47350.044.0967374
GCTGGGA148700.043.5270589
CCCGAGT25350.043.4196321
TTGGGAG116100.042.230436
TACTCGG21750.041.378923
GTAGCTG78300.041.2852756
CTACTCG22100.039.7462352
TAGCTGG87450.039.2707447
GGAGGCT125100.038.09889
GGGAGGC154750.037.6381658
TCGGGAG25700.037.2588236
AGGCGGA33550.037.126334
GCTACTC53900.035.1992231
CTCGGGA25850.035.0930065
GGCGGAG36500.034.9133075
GGAGGTT61450.034.5629468
Produced by FastQC (version 0.11.5)