FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867512
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19900.055.3230363
CCGAGTA20500.054.756842
GAGTAGC47850.050.2264184
AGTAGCT78550.050.1998065
GTAGCTG82150.047.737166
TTGGGAG116300.045.2896546
CGGAGGT25000.044.6219567
GCTGGGA153150.044.5970469
TAGCTGG92450.043.6729777
CCCGAGT27700.043.1217081
TACTCGG20450.040.1124533
GGAGGCT128000.039.3028569
GGGAGGC159100.038.8127148
GGAGGTT68150.038.2295888
CTACTCG21250.037.2478682
AGGCGGA38350.037.150744
TGGGAGG157950.036.498067
TTTGGGA121300.035.6517875
GCACTTT110500.035.2943081
TCGGGAG27750.035.2649776
Produced by FastQC (version 0.11.5)