FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5891071
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA24150.049.192362
CGAGTAG22850.047.894383
AGTAGCT90450.044.2559135
GAGTAGC58500.042.2148634
GTAGCTG96000.039.9724736
CCCGAGT34050.038.5155561
GCTGGGA174300.038.4967089
TTGGGAG129750.038.397576
TAGCTGG105950.037.7814757
CTACTCG23250.037.4708252
TACTCGG23850.036.2259673
GGGAGGC172500.033.639618
GGAGGCT139300.033.4393779
CGGAGGT26050.032.888527
GCTACTC58600.030.9112911
TGGGAGG176300.030.423497
GGAGGTT72950.030.19968
TTTGGGA138600.030.1279665
TCGGGAG32700.029.5027246
CTCGGGA29800.029.474655
Produced by FastQC (version 0.11.5)