FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5327211
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21050.059.136643
CCGAGTA21600.056.9645922
AGTAGCT84600.051.287275
GAGTAGC53550.051.0607034
GTAGCTG91450.045.871986
GCTGGGA171050.045.396059
CCCGAGT28400.045.3520931
CGGAGGT28700.042.627197
TACTCGG23600.042.380443
TAGCTGG105500.041.132457
TTGGGAG130800.040.4885946
CTACTCG23450.040.1967622
GGAGGCT136350.038.1595159
GGAGGTT72050.037.255868
GGGAGGC169000.037.1746838
CTCGGGA27700.035.587995
GCTACTC58600.035.3636861
TCGGGAG31950.035.1330956
AGGCGGA42150.034.3131944
TGGGAGG170550.033.6721537
Produced by FastQC (version 0.11.5)