FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5629048
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA20250.042.2841532
GAGTAGC48500.039.3151444
CGAGTAG19950.039.3132823
AGTAGCT76550.039.008445
TACTCGG21350.038.420473
CTACTCG21300.037.8350332
GCTGGGA149300.037.7445379
GTAGCTG78950.036.82016
CCCGAGT26800.036.5140341
TTGGGAG112800.036.296016
TAGCTGG90850.033.9847837
GGAGGCT129650.033.8617559
CGGAGGT24750.033.731447
GCTACTC53000.033.66911
CTCGGGA25750.033.532025
GGGAGGC154700.032.1004378
TCGGGAG28300.030.2563296
AGGCGGA39050.030.0346284
TGGGAGG149800.029.9795367
GGAGGTT66050.029.5289928
Produced by FastQC (version 0.11.5)