FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5649157
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21400.042.730862
AGTAGCT83850.041.5573545
GAGTAGC52350.040.9867634
CGAGTAG21000.040.801083
GTAGCTG85900.039.6436426
TTGGGAG126850.039.445726
GCTGGGA165200.038.220399
CGGAGGT26100.037.239077
TAGCTGG98600.036.1438037
TACTCGG23600.035.390773
GGAGGCT136550.035.3782549
GGAGGTT68650.035.132568
CCCGAGT29750.035.0808221
GGGAGGC167450.034.611178
CTACTCG23950.033.9722752
TGGGAGG169100.031.9743617
CTCGGGA26650.031.6077965
TCGGGAG29750.031.2182316
GAGGTTG91350.030.9734469
GCTACTC57500.029.998761
Produced by FastQC (version 0.11.5)