FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5649157
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT87800.044.992145
CCGAGTA22750.044.912352
GAGTAGC55450.043.3414844
GCTGGGA170600.043.1969229
CGAGTAG22300.042.5919883
TTGGGAG130150.041.740886
GTAGCTG94400.041.3891686
GGAGGCT137150.039.459719
TAGCTGG103400.039.0457887
CCCGAGT30000.038.855511
TACTCGG23750.038.7797663
GGGAGGC172800.037.8158578
CTACTCG23150.037.609122
GGAGGTT73850.035.6666648
GCTACTC55650.034.2641141
TGGGAGG171700.033.7409637
TCGGGAG31450.033.6322176
CTCGGGA29000.032.999895
GCACTTT123900.032.4057241
CGGAGGT28900.032.3725857
Produced by FastQC (version 0.11.5)