FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4240851
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22000.065.459393
AGTAGCT86200.065.230715
CCGAGTA23700.062.586912
GAGTAGC54250.062.38254
GTAGCTG91700.060.8472146
GCTGGGA180400.053.0790869
TTGGGAG133450.051.6838956
TAGCTGG107650.051.6311237
CCCGAGT30500.049.6298641
TACTCGG25950.048.5585063
CTACTCG25400.048.1925432
GCACTTT110700.044.162421
GGAGGCT141900.043.7861449
GGGAGGC182400.043.7344068
CAAGTAG43300.042.737573
TTTGGGA130500.042.5355645
TCGGGAG31350.042.256026
AAGTAGC45550.042.2072944
GCTACTC59800.040.4521871
CTCGGGA31900.039.9476175
Produced by FastQC (version 0.11.5)