FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4240851
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22000.067.7008743
CCGAGTA23450.064.435182
GAGTAGC54350.062.8840754
AGTAGCT86550.062.7662935
GTAGCTG93200.057.8245856
GCTGGGA180350.053.916169
TTGGGAG133300.053.006366
CCCGAGT31200.051.4276731
TAGCTGG109650.049.8866967
CTACTCG25400.048.157272
TACTCGG26300.045.962133
GCACTTT112450.045.4942741
TTTGGGA128100.045.328475
GGAGGCT144000.044.3344089
GGGAGGC192450.043.682228
GCTACTC61050.043.1362151
CAAGTAG45400.042.7912063
ACTTTGG118050.042.7875823
CACTTTG119750.042.120072
TCGGGAG32950.040.8350266
Produced by FastQC (version 0.11.5)