FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4088010
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT102650.084.749515
CCGAGTA29100.083.0837552
GAGTAGC67150.082.618674
CGAGTAG30000.081.070953
GTAGCTG111600.077.243596
TAGCTGG132600.066.432737
GCTGGGA231500.065.9998869
TTGGGAG156100.064.995926
CCCGAGT39550.063.1324731
GCACTTT124700.062.089141
CAAGTAG45150.061.0394863
TTTGGGA140750.059.6101235
CCAAGTA47900.059.3377952
AAGTAGC50250.058.1379624
ACTTTGG131450.057.6418083
AAGTGCT122550.057.4829755
CTGAGTA52250.057.152032
CACTTTG133350.056.5507162
TGAGTAG52700.056.254393
GGAGGCT172150.053.8877569
Produced by FastQC (version 0.11.5)