FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5970167
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG25400.032.8807563
CCGAGTA25850.032.597262
AGTAGCT99050.029.0752225
GAGTAGC64050.028.6641184
GTAGCTG107000.027.6551236
GCTGGGA198400.026.926499
TAGCTGG121500.026.0731777
CCCGAGT38200.025.8566131
TACTCGG28350.023.3642753
CTACTCG28300.022.6495382
TTGGGAG141000.021.8545676
GCTACTC68400.021.8186931
AGCTGGG170850.019.8061668
GGGAGGC196800.019.5724968
GGAGGCT160450.019.4745549
CTACTTG53200.018.9527472
CAAGTAG51800.018.9028243
CCCAAAG157350.018.7858811
AAGTGCT126800.018.6807445
CTCGGGA33900.018.4772425
Produced by FastQC (version 0.11.5)